Enzyme-linked immunosorbent assays (ELISA) complement antibody specificity with general enzyme sensitivity assays. This is done using an easy-to-perform test to detect antibodies or antigens. Antibody or antigen concentration is measured by ELISA. Enzyme-conjugated antibodies are the most flexible to detect.
MPO ELISA Kit is also one of the best kits available in the marketplace.
There are differences in these methods:
• ELISA can detect the presence of antigens recognized by antibodies.
• Used to test for antibodies that recognize antigens in samples.
• There are also several combination antibodies and antigen detection kits available.
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Steps for the ELISA procedure:
1. Primary antigen (or antibody) is used to cover the holes of the microtiter plate
2. Plates are washed to remove unbound antibodies. False-positive results are minimized by blocking unrelated sites (usually with bovine serum albumin or other animal proteins).
3. Addition of selected enzyme-conjugated primary detection antibody.
4. Addition of an enzyme-conjugated secondary antibody (such as anti-mouse IgG) Reacts this enzyme with any substrate present to produce a colored product indicating a positive reaction.
The results were measured by a spectrophotometer. Some analyzes include a sampling monitoring system that shows color changes at each step of the process. This can be important when using an automated liquid purification system as it can indicate at which stage the process is being interrupted or no reagents have been added.